(226) Mito-Q, a Mitochondrial-targeted Antioxidant, Partially Improves Relaxation of the Corpus Cavernosum in Aged Diabetic Mice

Abstract Introduction Erectile dysfunction is associated with increased contraction and decreased relaxation of the corpus cavernosum (CC). Excessive reactive oxygen species produced by mitochondria play a key role in aging- diabetes-induced erectile dysfunction. cavernosum. Mitochondrial deterioration part normal physiological aging, it plays pathogenesis age-related disorders producing (ROS). It also known that excessive ROS Objective We aimed to test hypothesis vitro inhibition mitochondrial oxidative stress improves reactivity CC. Methods To this hypothesis, CC young (3 4-month-old) old (10 14-month-old) male lean diabetic (dbdb-/-) mice were mounted myographs. Concentration-response curves acetylcholine (ACh 1 nM – 30 uM) induced electrical field stimulation (EFS 1, 2, 4, 8 16 Hz) obtained. All repeated after minutes incubation antioxidant Mito-Q (100 nM). Results Results: Relaxation ACh was significantly reduced (Lean: 87 ± 8% vs dbdb-/-: 60 6%). Similarly, aged presented impaired (Lean old: 54 6%) while displayed further decrease relaxing response (35 After Mito-Q, improved (62 3%) (44 1%). Relaxing responses EFS obese compared animals. However, no improvement seen Mito-Q. Conclusions Impairment endothelium-dependent aging diabetes production species. The use mitochondrial-targeted at least both mice. Disclosure No